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# The Fluid Mosaic Model of the Cell Membrane - The Mosaic

Module by: Laura Martin. E-mail the author

Although the bilayer nature of the cell membrane was described in the mid-1920's, it was not until 1972 that the currently accepted model of the plasma membrane, the fluid mosaic model, was formally outlined by S. J. Singer and Garth L. Nicolson in the journal Science.

Singer’s work on membrane structure originated in the 1950’s when he, along with other protein chemists, demonstrated that many water-soluble proteins like those found in cytoplasm could unexpectedly dissolve in nonaqueous, non-polar solvents. Furthermore, the shape a protein assumed differed in hydrophobic and hydrophilic environments (Singer, 1992).

From an historical perspective these results are significant because they led Singer to wonder about the structure of the proteins revealed to be closely associated with lipid-rich, and therefore nonaqueous, cell membranes in the 1930’s (Eichman, 2007). As he later wrote,

Although we had not experimented with membrane proteins and knew very little about membranes at the time,
as almost an aside we speculated [in a 1962 publication] that because “the cellular environment of many
proteins contains high concentrations of lipid components in a wide variety of cellular membranes, the
gross conformations of these proteins in situ may be determined by this association with a nonaqueous
environment.” This notion set off a train of ideas and experiments that eventually led us to the fluid
mosaic model. (Singer, 1992, p.3)


At the time Singer's train set off, the standard model for membrane structure, the Davson-Danielli-Roberston (DDR) model, was a bilayer of lipids sequestered between two monolayers of unfolded protein (Figure 1). Each protein layer faced an aqueous environment, cytoplasm or interstitial fluid, depending upon whether the membrane enclosed an organelle or the cell itself (Figure 1; Singer, 1992).

When Singer and colleagues applied their understanding of the influence of solvent environment on protein conformation specifically to the problem of membrane proteins, they realized the DDR model was energetically untenable. As he and Nicolson (1972) later wrote,

The latter [DDR] model is thermodynamically unstable because not only are the
non-polar amino acid residues of the membrane proteins in this model perforce [by circumstance]
largely exposed to water but the ionic and polar groups of the lipid are sequestered by a layer
of protein from contact with water. Therefore, neither hydrophobic nor hydrophilic interactions are
maximized in the classical [DDR] model. (Singer and Nicolson, 1972, p.721)


That is, the DDR model was energetically unfeasible because the constitutive molecules could not stably persist in aqueous cytoplasm in the physical conformation proposed. Just as oil and water will spontaneously separate when left to stand after shaking, the hydrophilic and hydrophobic components of a single polypeptide or an entire cell will spontaneously organize so that hydrophilic elements are in contact with the aqueous environment and the hydrophobic elements are sequestered, isolated from contact with polar components.

Thus, they reasoned that membrane proteins in a cell will assume globular (folded) conformations, due to hydrophobic and hydrophilic amino acid residues interacting with each other and the solvent environment, not the unfolded structures suggested by the DDR model. Similarly, membrane proteins will not be positioned to prevent contact between the polar head groups of membrane lipids and the aqueous cytoplasm.

So, if membrane proteins are globular and not layered on top of the membrane, where are they? How are they associated with the membrane?

The mosaic element of Singer and Nicolson's (1972) fluid mosaic model answered these questions. According to this model, membrane proteins come in two forms: peripheral proteins, which are dissolved in the cytoplasm and relatively loosely associated with the surface of the membrane, and integral proteins, which are integrated into the lipid matrix itself, to create a protein-phospholipid mosaic (Figure 2; Singer and Nicolson, 1972).

Singer and Nicolson (1972) supported these categories of proteins and their physical arrangement with both physical and biochemical evidence. For example, researchers had successfully separated the bilayers of frozen plasma membranes from a variety of sources including vacuoles, nuclei, chloroplasts, mitochondria and bacteria to reveal proteins embedded within (Singer and Nicolson, 1972). Similarly, evidence had also emerged to support the existence of transmembrane proteins, proteins that traversed the entire plasma membrane and extended into the aqueous environment on either side of the membrane (Figure 2).

Clear data supporting the predicted biochemical structure of integral proteins was harder to gain, however, and would only follow many years after the publication of the model. What was the biochemical structure of these proteins predicted to be?

Consider the energetic principles and molecular interactions on which Singer and Nicolson's model is based. Use your understanding of how these principles influence the structure and organization of individual polypeptides and the structural components of cells to answer the following questions.

1. Examine Figure 2. Predict biochemical properties (for example the hydrophobic or hydrophilic regions) of an integral protein versus those of a peripheral protein. Please be sure to explain your reasoning.

2. How do your predictions of the biochemical nature of integral proteins compare to Singer and Nicolson's predictions (Figure 3) adapted from a figure published by Lenard and Singer in 1966? If your predictions differ, please be sure to explain how and why they do.

## Works Cited

• Eichman, P. 2007. http://www1.umn.edu/ships/9-2/membrane.htm. SHiPS Resource Center for Sociology, History and Philosophy in Science Teaching
• Lenard, J. and S.J. Singer. 1966. Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism. Proceedings of the National Academy of Sciences. 56:1828-1835.
• Singer, S.J. and G. L. Nicolson. 1972. The fluid mosaic model of the structure of cell membranes. Science. 175: 720-731.
• Singer, S.J. 1992. The structure and function of membranes - a personal memoir. Journal of Membrane Biology. 129:3-12.

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