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Multiple Choice Questions

Module by: Professor Ho Huynh Thuy Duong. E-mail the author

  1. 1. One can distinguish prokaryotic chromosomes from eukaryotic chromosomes by determining:
    • a. Nucleotide sequence
    • b. Chromosome-linked proteins
    • c. Base composition
    • d. Secondary structure
  2. 2. In E. coli DNA replication, primer is:
    • a. A deoxyribonucleotide short sequence
    • b. A short RNA annealing to the 3’ end of the template strand
    • c. A short RNA complementary to the 5’ end of the leading strand
    • d. Synthesized by DNA polymerase I
  3. 3. Shine-Dalgarno sequence is:
    • a. Found at the 3’ end of a prokaryotic gene
    • b. Found in 16S rRNA
    • c. Complementary to an mRNA sequence
    • d. Located upstream of the AUG initiation codon of a prokaryotic mRNA
  4. 4. If the uracil content is exhausted, the following process will immediately stop:
    • a. Reverse transcription
    • b. Transcription
    • c. Replication
    • d. Translation
  5. 5. The promoter is:
    • a. A factor involving in translational process
    • b. Associated with repressor in an inducible operon
    • c. A sequence located at the 3’ end of a gene
    • d. The binding site for RNA polymerase
  6. 6. Proofreading activity of DNA polymerase III relies on:
    • a. The Mut S, H, L repair system recognizing parental DNA methylation
    • b. 3’-5’ exonuclease function of DNA polymerase
    • c. RNAse H activity
    • d. The UvrABC repair system
  7. 7. The difference on the regulation of gene expression in prokaryotes and eukaryotes is partly due to:
    • a. Different environmental conditions
    • b. Different cell components
    • c. Different cell structural features
    • d. Different cell numbers
  8. 8. The enzyme catalyzing the binding of Alanine to its tRNA is called:
    • a. Alanine-tRNA polymerase
    • b. Alanine-tRNA transferase
    • c. tRNA-Alanyl polymerase
    • d. Alanyl-tRNA synthetase
  9. 9. Microarray analysis can be used to:
    • a. Determine the intron-exon organization of a gene
    • b. Determine the concentration of a protein in a cell
    • c. Determine the stage-specific expression of a gene
    • d. Determine the presence of a DNA sequence in a cell
  10. 10. Hyperchromicity (increased OD value) results from:
    • a. Increased light absorbance by double-stranded DNA when it is denatured
    • b. Increased light absorbance by double-stranded DNA when it is hydrolyzed
    • c. Increased light absorbance by double-stranded DNA contaminated by RNA
    • d. Increased light absorbance by double-stranded DNA when it is renatured
  11. 11. The repair system acting just after the replication finishes is based on:
    • a. The elimination of methylated bases
    • b. The activities of Methylases
    • c. The recognition of hemimethylated DNA strands to be repaired
    • d. The excision of the oligonucleotide bearing the mismatch
  12. 12. The control of gene expression through an operon aims at:
    • a. Regulating different gene networks depending on the external stimuli
    • b. Regulating stepwise expression of a gene
    • c. Exerting a synchronous and fast regulation of genes belonging to one metabolism process
    • d. Producing different concentration of proteins of a metabolism process
  13. 13. Muscle, skin, liver cells differ from each other due to:
    • a. Different mutations arisen in each cell type
    • b. Different expression of genes in each cell type
    • c. Different genes present in different cell types
    • d. Different location of cell types in the organism
  14. 14. Automatic sequencing is based on:
    • a. The utilisation of fluorescent labeling
    • b. The utilisation of four types of dideoxynucleotide
    • c. The utilisation of DNA polymerases
    • d. All of the above items
  15. 15. Which of the following processes is involved in DNA repair:
    • a. Conjugation
    • b. Reversion of mutation
    • c. Transposition
    • d. Homologous recombination
  16. 16. Which of the following processes is characteristic to eukaryotic gene expression control:
    • a. Alternative splicing
    • b. Alternative use of σ factor
    • c. Transcription initiation
    • d. Catabolite repression

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