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Sample Critical Reading of Scientific Literature - Dr Gayle Slaughter

Module by: Dr. Sujata Krishna. E-mail the author

Summary: p53 activates transcription of p21 promoter without modification. P300 does not act by acetylating p53 itself, but acetylates p53 bound nucleosomes.

Transcriptional Regulation by p53

Molecular Cell 8:57

Summary: p53 activates transcription of p21 promoter without modification. P300 does not act by acetylating p53 itself, but acetylates p53 bound nucleosomes.

PositivesNegatives

Experimental results

(+Good purify, P300 active, Clear activation).1. Exp., purify., characterization p53 and p300 (-resolution histones)

2. In vitro translation and chromatin quality

(+ good controls) (-?chromatin C. l 7-9)

(+clear trancription) (-sizes from Mnase not given)

3. p300 affect on p53 dependent transcription

(+good controls)

(+ clear footprint)

4-5. role of C terminal in p53 dependent transcription

(+EMSA results clear)

6. p53 DNA binding affinity

7. mutant versus wt p53 dependent transcription

Conclusions

p53 functions synergistically with p300 to activate transcription

p53 does not require modification to be active C terminus is not inhibitory

p53 binds chromatin at higher affinity than DNA

p300 acetylates p53 bound nuclesomes

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